Primers Available
Organism:Huperzia squarrosa mitochondrion
Repeat:(GAAAA)3
Start:141429 End:141443
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AAGGCCAAATGAATTGATGG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AAAACAAGGATTGCCACGAC | |||||||||||||
| Tm_F_1 | 59.762 | Size_F_1 | 20 | Tm_R_1 | 59.978 | Size_R_1 | 20 | PRODUCT Size | 221 bp | Primer Start | 141256 | Primer End | 141476 |
| FORWARD_PRIMER_2 | |||||||||||||
| TAGATGCATCGCGTTGGTAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GATTTCAAAGGAACGACGGA | |||||||||||||
| Tm_F_2 | 60.237 | Size_F_2 | 20 | Tm_R_2 | 60.051 | Size_R_2 | 20 | PRODUCT Size | 241 bp | Primer Start | 141391 | Primer End | 141631 |
| FORWARD_PRIMER_3 | |||||||||||||
| AGGCCAAATGAATTGATGGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AAAACAAGGATTGCCACGAC | |||||||||||||
| Tm_F_3 | 60.274 | Size_F_3 | 20 | Tm_R_3 | 59.978 | Size_R_3 | 20 | PRODUCT Size | 220 bp | Primer Start | 141257 | Primer End | 141476 |