Primers Available
Organism:Huperzia squarrosa mitochondrion
Repeat:(G)11
Start:245385 End:245395
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTCTTGGACGATTTTCTGGG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ATACCCGACCACGTGAGAAG | |||||||||||||
| Tm_F_1 | 60.044 | Size_F_1 | 20 | Tm_R_1 | 59.989 | Size_R_1 | 20 | PRODUCT Size | 278 bp | Primer Start | 245323 | Primer End | 245600 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTCTTGGACGATTTTCTGGG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGGTTTGAAAATGCAAGACG | |||||||||||||
| Tm_F_2 | 60.044 | Size_F_2 | 20 | Tm_R_2 | 59.706 | Size_R_2 | 20 | PRODUCT Size | 132 bp | Primer Start | 245323 | Primer End | 245454 |
| FORWARD_PRIMER_3 | |||||||||||||
| AGGGGTGTTTTTCGTTCTCC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ATACCCGACCACGTGAGAAG | |||||||||||||
| Tm_F_3 | 60.34 | Size_F_3 | 20 | Tm_R_3 | 59.989 | Size_R_3 | 20 | PRODUCT Size | 241 bp | Primer Start | 245360 | Primer End | 245600 |