Primers Available
Organism:Huperzia squarrosa mitochondrion
Repeat:(C)10
Start:173081 End:173090
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GTAAGGCAGACCTTTGCGAG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GAACCTTTGGGACGAATCAA | |||||||||||||
| Tm_F_1 | 60.015 | Size_F_1 | 20 | Tm_R_1 | 59.91 | Size_R_1 | 20 | PRODUCT Size | 248 bp | Primer Start | 172890 | Primer End | 173137 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTGTTTTCGTTTCTCGGACC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTTCGGACTACATTCTCGGG | |||||||||||||
| Tm_F_2 | 60.088 | Size_F_2 | 20 | Tm_R_2 | 60.066 | Size_R_2 | 20 | PRODUCT Size | 263 bp | Primer Start | 172970 | Primer End | 173232 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCGGTTTGGCTTTTGTTTTC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTTCGGACTACATTCTCGGG | |||||||||||||
| Tm_F_3 | 60.089 | Size_F_3 | 20 | Tm_R_3 | 60.066 | Size_R_3 | 20 | PRODUCT Size | 275 bp | Primer Start | 172958 | Primer End | 173232 |