Primers Available
Organism:Amblyomma sphenodonti mitochondrion
Repeat:(T)10(TTTA)3*
Start:13415 End:13433
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ATCTCCACTGCGTTTTCCAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GATATTTGTCCCCAAGGCAA | |||||||||||||
| Tm_F_1 | 59.556 | Size_F_1 | 20 | Tm_R_1 | 59.762 | Size_R_1 | 20 | PRODUCT Size | 242 bp | Primer Start | 13323 | Primer End | 13564 |
| FORWARD_PRIMER_2 | |||||||||||||
| ATCTCCACTGCGTTTTCCAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTTGTCCCCAAGGCAAAATA | |||||||||||||
| Tm_F_2 | 59.556 | Size_F_2 | 20 | Tm_R_2 | 60.294 | Size_R_2 | 20 | PRODUCT Size | 238 bp | Primer Start | 13323 | Primer End | 13560 |
| FORWARD_PRIMER_3 | |||||||||||||
| TATCTCCACTGCGTTTTCCA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GATATTTGTCCCCAAGGCAA | |||||||||||||
| Tm_F_3 | 59.272 | Size_F_3 | 20 | Tm_R_3 | 59.762 | Size_R_3 | 20 | PRODUCT Size | 243 bp | Primer Start | 13322 | Primer End | 13564 |