Primers Available
Organism:Amblyomma sphenodonti mitochondrion
Repeat:(AATTT)3
Start:4969 End:4983
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCAAGCTCAATTTTCCATTTC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CTGAAGCCTCAAACCCAAAG | |||||||||||||
| Tm_F_1 | 58.683 | Size_F_1 | 21 | Tm_R_1 | 59.846 | Size_R_1 | 20 | PRODUCT Size | 170 bp | Primer Start | 4846 | Primer End | 5015 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCAAGCTCAATTTTCCATTTCT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CTGAAGCCTCAAACCCAAAG | |||||||||||||
| Tm_F_2 | 59.609 | Size_F_2 | 22 | Tm_R_2 | 59.846 | Size_R_2 | 20 | PRODUCT Size | 170 bp | Primer Start | 4846 | Primer End | 5015 |
| FORWARD_PRIMER_3 | |||||||||||||
| ACCAAGCTCAATTTTCCATTTC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CTGAAGCCTCAAACCCAAAG | |||||||||||||
| Tm_F_3 | 59.485 | Size_F_3 | 22 | Tm_R_3 | 59.846 | Size_R_3 | 20 | PRODUCT Size | 171 bp | Primer Start | 4845 | Primer End | 5015 |