Primers Available
Organism:Amblyomma sphenodonti mitochondrion
Repeat:(A)12
Start:9873 End:9884
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGCGCATGCAATATAAAAATG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AAGGGGATTCAGGATGTACG | |||||||||||||
| Tm_F_1 | 59.593 | Size_F_1 | 21 | Tm_R_1 | 58.861 | Size_R_1 | 20 | PRODUCT Size | 226 bp | Primer Start | 9799 | Primer End | 10024 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGCGCATGCAATATAAAAATG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AAAGGGGATTCAGGATGTACG | |||||||||||||
| Tm_F_2 | 59.593 | Size_F_2 | 21 | Tm_R_2 | 60.194 | Size_R_2 | 21 | PRODUCT Size | 227 bp | Primer Start | 9799 | Primer End | 10025 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGCGCATGCAATATAAAAATG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGATTTCCTTTCATTGGAGG | |||||||||||||
| Tm_F_3 | 59.593 | Size_F_3 | 21 | Tm_R_3 | 58.427 | Size_R_3 | 20 | PRODUCT Size | 133 bp | Primer Start | 9799 | Primer End | 9931 |