Primers Available
Organism:Cirrhinus mrigala mitochondrion
Repeat:(TA)12
Start:805 End:828
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGTTTTCCTTGTCAAACCCC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTGTGCATGCGGAGTTTTT | |||||||||||||
| Tm_F_1 | 59.807 | Size_F_1 | 20 | Tm_R_1 | 60.25 | Size_R_1 | 19 | PRODUCT Size | 275 bp | Primer Start | 719 | Primer End | 993 |
| FORWARD_PRIMER_2 | |||||||||||||
| AAGGAGGACTCGAGAACGTG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CTTTGTGCATGCGGAGTTT | |||||||||||||
| Tm_F_2 | 59.45 | Size_F_2 | 20 | Tm_R_2 | 59.856 | Size_R_2 | 19 | PRODUCT Size | 251 bp | Primer Start | 745 | Primer End | 995 |
| FORWARD_PRIMER_3 | |||||||||||||
| AAGGAGGACTCGAGAACGTG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CTTTGTGCATGCGGAGTTTT | |||||||||||||
| Tm_F_3 | 59.45 | Size_F_3 | 20 | Tm_R_3 | 61.196 | Size_R_3 | 20 | PRODUCT Size | 251 bp | Primer Start | 745 | Primer End | 995 |