Primers Available
Organism:Pentactina rupicola chloroplast
Repeat:(T)10ctaggtgaaatgcctag(A)10
Start:70544 End:70580
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCCTTCCCGTGTCCTAGTCT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AAAGAAGGGCTCCGGTGTAT | |||||||||||||
| Tm_F_1 | 60.502 | Size_F_1 | 20 | Tm_R_1 | 59.962 | Size_R_1 | 20 | PRODUCT Size | 279 bp | Primer Start | 70436 | Primer End | 70714 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCAGTTATTCCCTTCCCGTG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CCATAGAAACGATGGAACCC | |||||||||||||
| Tm_F_2 | 59.926 | Size_F_2 | 20 | Tm_R_2 | 59.244 | Size_R_2 | 20 | PRODUCT Size | 240 bp | Primer Start | 70427 | Primer End | 70666 |
| FORWARD_PRIMER_3 | |||||||||||||
| AAAATTCCAATGGCTTTTGCT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AAAGAAGGGCTCCGGTGTAT | |||||||||||||
| Tm_F_3 | 59.963 | Size_F_3 | 21 | Tm_R_3 | 59.962 | Size_R_3 | 20 | PRODUCT Size | 222 bp | Primer Start | 70493 | Primer End | 70714 |