Primers Available
Organism:Pentactina rupicola chloroplast
Repeat:(AATAA)4
Start:31727 End:31746
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ATTGAATTTGGATTCGTCGG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGGCTCTGTTACAACACGCT | |||||||||||||
| Tm_F_1 | 59.762 | Size_F_1 | 20 | Tm_R_1 | 59.515 | Size_R_1 | 20 | PRODUCT Size | 200 bp | Primer Start | 31622 | Primer End | 31821 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCGTTGGGAATGAAATTCTG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGGCTCTGTTACAACACGCT | |||||||||||||
| Tm_F_2 | 60.301 | Size_F_2 | 20 | Tm_R_2 | 59.515 | Size_R_2 | 20 | PRODUCT Size | 273 bp | Primer Start | 31549 | Primer End | 31821 |
| FORWARD_PRIMER_3 | |||||||||||||
| ATTGAATTTGGATTCGTCGG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TAGTAATCCAGGCCGCTCTC | |||||||||||||
| Tm_F_3 | 59.762 | Size_F_3 | 20 | Tm_R_3 | 59.434 | Size_R_3 | 20 | PRODUCT Size | 268 bp | Primer Start | 31622 | Primer End | 31889 |