Primers Available
Organism:Pentactina rupicola chloroplast
Repeat:(A)14tcaaaagccagacccagtgtagtgcagtacggtacggtatcccgcggaatcctcaatccgatgc(TAA)4
Start:31327 End:31416
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ATCGGGCCAGAAAAGGATA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTGATGGGAGAGAGACCCAT | |||||||||||||
| Tm_F_1 | 59.472 | Size_F_1 | 19 | Tm_R_1 | 59.46 | Size_R_1 | 20 | PRODUCT Size | 280 bp | Primer Start | 31178 | Primer End | 31457 |
| FORWARD_PRIMER_2 | |||||||||||||
| ACTCCCGGGAAACATACAGT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ATTGATGGGAGAGAGACCCA | |||||||||||||
| Tm_F_2 | 58.377 | Size_F_2 | 20 | Tm_R_2 | 59.46 | Size_R_2 | 20 | PRODUCT Size | 187 bp | Primer Start | 31272 | Primer End | 31458 |
| FORWARD_PRIMER_3 | |||||||||||||
| ACTCCCGGGAAACATACAGT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTGATGGGAGAGAGACCCAT | |||||||||||||
| Tm_F_3 | 58.377 | Size_F_3 | 20 | Tm_R_3 | 59.46 | Size_R_3 | 20 | PRODUCT Size | 186 bp | Primer Start | 31272 | Primer End | 31457 |