Primers Available
Organism:Seriola quinqueradiata mitochondrion
Repeat:(CCT)4
Start:10876 End:10887
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GCCGGGACTTACTTCCTCTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ACTATTGATCCTGCGATGGG | |||||||||||||
| Tm_F_1 | 59.713 | Size_F_1 | 20 | Tm_R_1 | 59.917 | Size_R_1 | 20 | PRODUCT Size | 257 bp | Primer Start | 10819 | Primer End | 11075 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCAGTAAGCCGACAACGAAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AGGGTTAGGAGGGAGAGGGT | |||||||||||||
| Tm_F_2 | 60.132 | Size_F_2 | 20 | Tm_R_2 | 60.316 | Size_R_2 | 20 | PRODUCT Size | 275 bp | Primer Start | 10648 | Primer End | 10922 |
| FORWARD_PRIMER_3 | |||||||||||||
| CAATTCTTCCTGATCCTGGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CGTAGCTTGCAAGGGGTAAG | |||||||||||||
| Tm_F_3 | 59.629 | Size_F_3 | 20 | Tm_R_3 | 59.898 | Size_R_3 | 20 | PRODUCT Size | 265 bp | Primer Start | 10693 | Primer End | 10957 |