Primers Available
Organism:Seriola quinqueradiata mitochondrion
Repeat:(AACA)3
Start:13517 End:13528
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCTTCCTTTAAAAACCCCCA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGCGATGAATAATTGACGGG | |||||||||||||
| Tm_F_1 | 60.15 | Size_F_1 | 20 | Tm_R_1 | 59.923 | Size_R_1 | 20 | PRODUCT Size | 194 bp | Primer Start | 13411 | Primer End | 13604 |
| FORWARD_PRIMER_2 | |||||||||||||
| ATCAAACGATTAGCATGGGG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AGCGATGAATAATTGACGGG | |||||||||||||
| Tm_F_2 | 59.784 | Size_F_2 | 20 | Tm_R_2 | 59.923 | Size_R_2 | 20 | PRODUCT Size | 250 bp | Primer Start | 13355 | Primer End | 13604 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCTTCCTTTAAAAACCCCCA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTAGAAAAATGGTGGGTGGC | |||||||||||||
| Tm_F_3 | 60.15 | Size_F_3 | 20 | Tm_R_3 | 59.801 | Size_R_3 | 20 | PRODUCT Size | 159 bp | Primer Start | 13411 | Primer End | 13569 |