Primers Available
Organism:Lotus japonicus mitochondrion
Repeat:(AACT)3
Start:270306 End:270317
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCCAACATCAACTCTCCGTG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGACTCTAGAAGCTCGCCCC | |||||||||||||
| Tm_F_1 | 59.676 | Size_F_1 | 20 | Tm_R_1 | 60.117 | Size_R_1 | 20 | PRODUCT Size | 129 bp | Primer Start | 270273 | Primer End | 270401 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTGAGCAGCCAGCTATCAGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGCTCTTAGCGCCTCTCTCT | |||||||||||||
| Tm_F_2 | 59.847 | Size_F_2 | 20 | Tm_R_2 | 59.621 | Size_R_2 | 20 | PRODUCT Size | 239 bp | Primer Start | 270122 | Primer End | 270360 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCCAACATCAACTCTCCGTG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGTTGCAGCTTATCCATCCA | |||||||||||||
| Tm_F_3 | 59.676 | Size_F_3 | 20 | Tm_R_3 | 60.22 | Size_R_3 | 20 | PRODUCT Size | 215 bp | Primer Start | 270273 | Primer End | 270487 |