Primers Available
Organism:Boea hygrometrica mitochondrion
Repeat:(TGAC)3
Start:506722 End:506733
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTGAATGACTGGCTTGCTTG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGCGCAGTAGAATAGAGCGG | |||||||||||||
| Tm_F_1 | 59.988 | Size_F_1 | 20 | Tm_R_1 | 59.775 | Size_R_1 | 20 | PRODUCT Size | 230 bp | Primer Start | 506681 | Primer End | 506910 |
| FORWARD_PRIMER_2 | |||||||||||||
| GGTACCTCTCGTCGAACTGG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CGGTGATGCTACGACGAGTA | |||||||||||||
| Tm_F_2 | 59.721 | Size_F_2 | 20 | Tm_R_2 | 59.888 | Size_R_2 | 20 | PRODUCT Size | 276 bp | Primer Start | 506485 | Primer End | 506760 |
| FORWARD_PRIMER_3 | |||||||||||||
| GGTACCTCTCGTCGAACTGG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TATCGGTGATGCTACGACGA | |||||||||||||
| Tm_F_3 | 59.721 | Size_F_3 | 20 | Tm_R_3 | 60.243 | Size_R_3 | 20 | PRODUCT Size | 279 bp | Primer Start | 506485 | Primer End | 506763 |