Primers Available
Organism:Boea hygrometrica mitochondrion
Repeat:(ACTA)3
Start:203484 End:203495
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCCTAAGCTAGGGGAGAGGG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGGTACAGATCCGCATGTCC | |||||||||||||
| Tm_F_1 | 59.799 | Size_F_1 | 20 | Tm_R_1 | 59.957 | Size_R_1 | 20 | PRODUCT Size | 149 bp | Primer Start | 203435 | Primer End | 203583 |
| FORWARD_PRIMER_2 | |||||||||||||
| ACGGTAAGAGACCTGGACCC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AGGTACAGATCCGCATGTCC | |||||||||||||
| Tm_F_2 | 60.371 | Size_F_2 | 20 | Tm_R_2 | 59.957 | Size_R_2 | 20 | PRODUCT Size | 258 bp | Primer Start | 203326 | Primer End | 203583 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCCTAAGCTAGGGGAGAGGG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CCATAAATCAGGCAATCGGT | |||||||||||||
| Tm_F_3 | 59.799 | Size_F_3 | 20 | Tm_R_3 | 59.784 | Size_R_3 | 20 | PRODUCT Size | 131 bp | Primer Start | 203435 | Primer End | 203565 |