Primers Available
Organism:Synedra acus chloroplast
Repeat:(TTA)4
Start:2829 End:2840
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTTGGGATCCACATTTTGGT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCAACCAGCAAATAATAAAGCA | |||||||||||||
| Tm_F_1 | 60.029 | Size_F_1 | 20 | Tm_R_1 | 59.173 | Size_R_1 | 22 | PRODUCT Size | 188 bp | Primer Start | 2679 | Primer End | 2866 |
| FORWARD_PRIMER_2 | |||||||||||||
| AATCGCTCATGCTATTTGGG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CCAACCAGCAAATAATAAAGCA | |||||||||||||
| Tm_F_2 | 60.06 | Size_F_2 | 20 | Tm_R_2 | 59.173 | Size_R_2 | 22 | PRODUCT Size | 202 bp | Primer Start | 2665 | Primer End | 2866 |
| FORWARD_PRIMER_3 | |||||||||||||
| CTCTGGTGTATACCAATGGTGG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ATGAACACCACGTGAAGCTG | |||||||||||||
| Tm_F_3 | 59.238 | Size_F_3 | 22 | Tm_R_3 | 59.751 | Size_R_3 | 20 | PRODUCT Size | 268 bp | Primer Start | 2755 | Primer End | 3022 |