Primers Available
Organism:Synedra acus chloroplast
Repeat:(GCT)4
Start:54735 End:54746
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACTGGGATGCTTCATACGCT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGGTCACTTGCATTTGGAAC | |||||||||||||
| Tm_F_1 | 59.723 | Size_F_1 | 20 | Tm_R_1 | 59.547 | Size_R_1 | 20 | PRODUCT Size | 217 bp | Primer Start | 54648 | Primer End | 54864 |
| FORWARD_PRIMER_2 | |||||||||||||
| ACTGGGATGCTTCATACGCT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGTAGCGCTCACAAGCTGTT | |||||||||||||
| Tm_F_2 | 59.723 | Size_F_2 | 20 | Tm_R_2 | 60.602 | Size_R_2 | 20 | PRODUCT Size | 171 bp | Primer Start | 54648 | Primer End | 54818 |
| FORWARD_PRIMER_3 | |||||||||||||
| CGCTAAAATGGGTTACTGGG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ATTCGTAAGCGATGAAAGCG | |||||||||||||
| Tm_F_3 | 59.469 | Size_F_3 | 20 | Tm_R_3 | 60.364 | Size_R_3 | 20 | PRODUCT Size | 257 bp | Primer Start | 54634 | Primer End | 54890 |