Primers Available
Organism:Potamothrissa obtusirostris mitochondrial DNA
Repeat:(AACC)3
Start:8474 End:8485
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GCCTCTTAACCCAGGAGGAC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AACTGGGATTAAAGGGACGG | |||||||||||||
| Tm_F_1 | 60.074 | Size_F_1 | 20 | Tm_R_1 | 60.18 | Size_R_1 | 20 | PRODUCT Size | 244 bp | Primer Start | 8295 | Primer End | 8538 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCCTTCTGCCTCTTAACCCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGGGGTCCCTTCTGGTAGTA | |||||||||||||
| Tm_F_2 | 59.807 | Size_F_2 | 20 | Tm_R_2 | 60.183 | Size_R_2 | 20 | PRODUCT Size | 233 bp | Primer Start | 8288 | Primer End | 8520 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTCTGCCTCTTAACCCAGGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGGGGTCCCTTCTGGTAGTA | |||||||||||||
| Tm_F_3 | 59.807 | Size_F_3 | 20 | Tm_R_3 | 60.183 | Size_R_3 | 20 | PRODUCT Size | 230 bp | Primer Start | 8291 | Primer End | 8520 |