Primers Available
Organism:Gossypium herbaceum subsp. africanum chloroplast
Repeat:(T)10caattgaaaattccc(AAT)4
Start:13524 End:13560
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CGCTTTGACTCCTTTTCCAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGCCATTGACATGAAACCTG | |||||||||||||
| Tm_F_1 | 59.312 | Size_F_1 | 20 | Tm_R_1 | 60.517 | Size_R_1 | 20 | PRODUCT Size | 274 bp | Primer Start | 13325 | Primer End | 13598 |
| FORWARD_PRIMER_2 | |||||||||||||
| CGCTTTGACTCCTTTTCCAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GAAACCTGCCCCCTAATTCT | |||||||||||||
| Tm_F_2 | 59.312 | Size_F_2 | 20 | Tm_R_2 | 59.42 | Size_R_2 | 20 | PRODUCT Size | 262 bp | Primer Start | 13325 | Primer End | 13586 |
| FORWARD_PRIMER_3 | |||||||||||||
| CGCTTTGACTCCTTTTCCAT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTTGCCATTGACATGAAACC | |||||||||||||
| Tm_F_3 | 59.312 | Size_F_3 | 20 | Tm_R_3 | 59.375 | Size_R_3 | 20 | PRODUCT Size | 276 bp | Primer Start | 13325 | Primer End | 13600 |