Primers Available
Organism:Gossypium herbaceum subsp. africanum chloroplast
Repeat:(T)10
Start:131905 End:131914
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GTTCCGCGATGAAAATGAAC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGTAATCAAATGATCTCGCCG | |||||||||||||
| Tm_F_1 | 60.457 | Size_F_1 | 20 | Tm_R_1 | 59.196 | Size_R_1 | 21 | PRODUCT Size | 278 bp | Primer Start | 131846 | Primer End | 132123 |
| FORWARD_PRIMER_2 | |||||||||||||
| GTTCCGCGATGAAAATGAAC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GTAATCAAATGATCTCGCCG | |||||||||||||
| Tm_F_2 | 60.457 | Size_F_2 | 20 | Tm_R_2 | 58.187 | Size_R_2 | 20 | PRODUCT Size | 277 bp | Primer Start | 131846 | Primer End | 132122 |
| FORWARD_PRIMER_3 | |||||||||||||
| AGTTCCGCGATGAAAATGAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGTAATCAAATGATCTCGCCG | |||||||||||||
| Tm_F_3 | 60.585 | Size_F_3 | 20 | Tm_R_3 | 59.196 | Size_R_3 | 21 | PRODUCT Size | 279 bp | Primer Start | 131845 | Primer End | 132123 |