Primers Available
Organism:Gossypium herbaceum subsp. africanum chloroplast
Repeat:(A)12
Start:85492 End:85503
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCAAATGGGTCTGAGGTTGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCGTTAAACCGGGCAGAATA | |||||||||||||
| Tm_F_1 | 59.059 | Size_F_1 | 20 | Tm_R_1 | 60.448 | Size_R_1 | 20 | PRODUCT Size | 280 bp | Primer Start | 85406 | Primer End | 85685 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCAAATGGGTCTGAGGTTGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CGTTAAACCGGGCAGAATAC | |||||||||||||
| Tm_F_2 | 59.059 | Size_F_2 | 20 | Tm_R_2 | 59.474 | Size_R_2 | 20 | PRODUCT Size | 279 bp | Primer Start | 85406 | Primer End | 85684 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCAAATGGGTCTGAGGTTGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GAGCGGAGTAGCCGAAAATA | |||||||||||||
| Tm_F_3 | 59.059 | Size_F_3 | 20 | Tm_R_3 | 59.456 | Size_R_3 | 20 | PRODUCT Size | 249 bp | Primer Start | 85406 | Primer End | 85654 |