Primers Available
Organism:Gossypium herbaceum subsp. africanum chloroplast
Repeat:(A)10
Start:5660 End:5669
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CTCGTACGGCTCGAGAAAAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AAAGGGGTTAGAGACCACTCAA | |||||||||||||
| Tm_F_1 | 60.514 | Size_F_1 | 20 | Tm_R_1 | 59.144 | Size_R_1 | 22 | PRODUCT Size | 248 bp | Primer Start | 5524 | Primer End | 5771 |
| FORWARD_PRIMER_2 | |||||||||||||
| CTCGTACGGCTCGAGAAAAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AAAAGGGGTTAGAGACCACTCA | |||||||||||||
| Tm_F_2 | 60.514 | Size_F_2 | 20 | Tm_R_2 | 59.144 | Size_R_2 | 22 | PRODUCT Size | 249 bp | Primer Start | 5524 | Primer End | 5772 |
| FORWARD_PRIMER_3 | |||||||||||||
| GATCCATAAACCAGCAAATCAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CCCAATGATTCAAGGCAAAG | |||||||||||||
| Tm_F_3 | 58.946 | Size_F_3 | 22 | Tm_R_3 | 60.439 | Size_R_3 | 20 | PRODUCT Size | 280 bp | Primer Start | 5608 | Primer End | 5887 |