Primers Available
Organism:Gossypium herbaceum subsp. africanum chloroplast
Repeat:(A)10
Start:130700 End:130709
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ATATATGTGCTCCGCGATCC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGGAGGATTCTTATTTTCCCC | |||||||||||||
| Tm_F_1 | 59.914 | Size_F_1 | 20 | Tm_R_1 | 59.976 | Size_R_1 | 21 | PRODUCT Size | 169 bp | Primer Start | 130663 | Primer End | 130831 |
| FORWARD_PRIMER_2 | |||||||||||||
| ATATATGTGCTCCGCGATCC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGGGGAGGATTCTTATTTTCC | |||||||||||||
| Tm_F_2 | 59.914 | Size_F_2 | 20 | Tm_R_2 | 59.976 | Size_R_2 | 21 | PRODUCT Size | 171 bp | Primer Start | 130663 | Primer End | 130833 |
| FORWARD_PRIMER_3 | |||||||||||||
| ATATATGTGCTCCGCGATCC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CCATCGCCCATTTGTATTTG | |||||||||||||
| Tm_F_3 | 59.914 | Size_F_3 | 20 | Tm_R_3 | 61.074 | Size_R_3 | 20 | PRODUCT Size | 150 bp | Primer Start | 130663 | Primer End | 130812 |