Primers Available
Organism:Gossypium darwinii chloroplast
Repeat:(TTA)4
Start:8425 End:8436
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACGCAAAAAGAAAGGCTTGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTTGAGATATTTCTTTAGAGCCATTG | |||||||||||||
| Tm_F_1 | 59.996 | Size_F_1 | 20 | Tm_R_1 | 59.291 | Size_R_1 | 26 | PRODUCT Size | 268 bp | Primer Start | 8306 | Primer End | 8573 |
| FORWARD_PRIMER_2 | |||||||||||||
| ACGCAAAAAGAAAGGCTTGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTGAGATATTTCTTTAGAGCCATTG | |||||||||||||
| Tm_F_2 | 59.996 | Size_F_2 | 20 | Tm_R_2 | 58.144 | Size_R_2 | 25 | PRODUCT Size | 267 bp | Primer Start | 8306 | Primer End | 8572 |
| FORWARD_PRIMER_3 | |||||||||||||
| ACAGCACGCAAAAAGAAAGG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTTGAGATATTTCTTTAGAGCCATTG | |||||||||||||
| Tm_F_3 | 60.42 | Size_F_3 | 20 | Tm_R_3 | 59.291 | Size_R_3 | 26 | PRODUCT Size | 273 bp | Primer Start | 8301 | Primer End | 8573 |