Primers Available
Organism:Gossypium darwinii chloroplast
Repeat:(TAGT)3
Start:62528 End:62539
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CGATCCAATCGTACCACGTA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGTCACAAAGTCAAAGCCCA | |||||||||||||
| Tm_F_1 | 59.42 | Size_F_1 | 20 | Tm_R_1 | 60.278 | Size_R_1 | 20 | PRODUCT Size | 196 bp | Primer Start | 62393 | Primer End | 62588 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCTATATTGCATTTGCGGGT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CGGGCTTTGACTTTGATTCT | |||||||||||||
| Tm_F_2 | 59.816 | Size_F_2 | 20 | Tm_R_2 | 59.312 | Size_R_2 | 20 | PRODUCT Size | 274 bp | Primer Start | 62290 | Primer End | 62563 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCTATATTGCATTTGCGGGT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ATTACGCGGGCTTTGACTTT | |||||||||||||
| Tm_F_3 | 59.816 | Size_F_3 | 20 | Tm_R_3 | 60.976 | Size_R_3 | 20 | PRODUCT Size | 280 bp | Primer Start | 62290 | Primer End | 62569 |