Primers Available
Organism:Gossypium darwinii chloroplast
Repeat:(AAAT)3
Start:6515 End:6526
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GCACGTTGCTTTCTACCACA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTCAGACAATCATACAATATGGGG | |||||||||||||
| Tm_F_1 | 59.911 | Size_F_1 | 20 | Tm_R_1 | 59.97 | Size_R_1 | 24 | PRODUCT Size | 242 bp | Primer Start | 6346 | Primer End | 6587 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCATGTCCTTCAAGTCGCAC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTCAGACAATCATACAATATGGGG | |||||||||||||
| Tm_F_2 | 59.837 | Size_F_2 | 20 | Tm_R_2 | 59.97 | Size_R_2 | 24 | PRODUCT Size | 258 bp | Primer Start | 6330 | Primer End | 6587 |
| FORWARD_PRIMER_3 | |||||||||||||
| GCACGTTGCTTTCTACCACA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCAGACAATCATACAATATGGGG | |||||||||||||
| Tm_F_3 | 59.911 | Size_F_3 | 20 | Tm_R_3 | 58.707 | Size_R_3 | 23 | PRODUCT Size | 241 bp | Primer Start | 6346 | Primer End | 6586 |