Primers Available
Organism:Gossypium darwinii chloroplast
Repeat:(A)12
Start:145303 End:145314
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGGGACTTAGGGAATGATGG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AAACATCTGCGGATCCAATC | |||||||||||||
| Tm_F_1 | 59.744 | Size_F_1 | 20 | Tm_R_1 | 59.9 | Size_R_1 | 20 | PRODUCT Size | 248 bp | Primer Start | 145269 | Primer End | 145516 |
| FORWARD_PRIMER_2 | |||||||||||||
| AGGGAATGATGGAAGGGAAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AAACATCTGCGGATCCAATC | |||||||||||||
| Tm_F_2 | 59.588 | Size_F_2 | 20 | Tm_R_2 | 59.9 | Size_R_2 | 20 | PRODUCT Size | 240 bp | Primer Start | 145277 | Primer End | 145516 |
| FORWARD_PRIMER_3 | |||||||||||||
| TATTTCGTACCCCTCGCTCA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTCTCGATCAATCCCTTTGC | |||||||||||||
| Tm_F_3 | 60.597 | Size_F_3 | 20 | Tm_R_3 | 60.155 | Size_R_3 | 20 | PRODUCT Size | 255 bp | Primer Start | 145212 | Primer End | 145466 |