Primers Available
Organism:Gossypium darwinii chloroplast
Repeat:(A)11
Start:120356 End:120366
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GAAACCCGGATTGATTCATTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTTGAAATTCAGCAGACCCC | |||||||||||||
| Tm_F_1 | 60.013 | Size_F_1 | 21 | Tm_R_1 | 60.051 | Size_R_1 | 20 | PRODUCT Size | 225 bp | Primer Start | 120328 | Primer End | 120552 |
| FORWARD_PRIMER_2 | |||||||||||||
| ATCGAAACCCGGATTGATTC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTTGAAATTCAGCAGACCCC | |||||||||||||
| Tm_F_2 | 61.025 | Size_F_2 | 20 | Tm_R_2 | 60.051 | Size_R_2 | 20 | PRODUCT Size | 228 bp | Primer Start | 120325 | Primer End | 120552 |
| FORWARD_PRIMER_3 | |||||||||||||
| GAAACCCGGATTGATTCATTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGCAGACCCCCTGTTTTCTT | |||||||||||||
| Tm_F_3 | 60.013 | Size_F_3 | 21 | Tm_R_3 | 60.11 | Size_R_3 | 20 | PRODUCT Size | 215 bp | Primer Start | 120328 | Primer End | 120542 |