Primers Available
Organism:Gossypium darwinii chloroplast
Repeat:(A)10
Start:115478 End:115487
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GCGAATTCCACTTTCGTTTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CATTTTCAACCCGGTCATTT | |||||||||||||
| Tm_F_1 | 59.205 | Size_F_1 | 20 | Tm_R_1 | 59.662 | Size_R_1 | 20 | PRODUCT Size | 222 bp | Primer Start | 115374 | Primer End | 115595 |
| FORWARD_PRIMER_2 | |||||||||||||
| GGATCCGAGCAAAGAAACAG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ATATCGCAAAGGTCGATTCC | |||||||||||||
| Tm_F_2 | 59.813 | Size_F_2 | 20 | Tm_R_2 | 59.001 | Size_R_2 | 20 | PRODUCT Size | 259 bp | Primer Start | 115312 | Primer End | 115570 |
| FORWARD_PRIMER_3 | |||||||||||||
| GTGGATCCGAGCAAAGAAAC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ATATCGCAAAGGTCGATTCC | |||||||||||||
| Tm_F_3 | 59.676 | Size_F_3 | 20 | Tm_R_3 | 59.001 | Size_R_3 | 20 | PRODUCT Size | 261 bp | Primer Start | 115310 | Primer End | 115570 |