Primers Available
Organism:Gossypium raimondii chloroplast
Repeat:(TTTCT)3
Start:144025 End:144039
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GACCCTTTAAGACCCCGAAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CAATGGAGTTGGGCTTTCAT | |||||||||||||
| Tm_F_1 | 60.291 | Size_F_1 | 20 | Tm_R_1 | 59.933 | Size_R_1 | 20 | PRODUCT Size | 251 bp | Primer Start | 143994 | Primer End | 144244 |
| FORWARD_PRIMER_2 | |||||||||||||
| GACCCTTTAAGACCCCGAAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CAACATGGATTCTGGCAATG | |||||||||||||
| Tm_F_2 | 60.291 | Size_F_2 | 20 | Tm_R_2 | 59.924 | Size_R_2 | 20 | PRODUCT Size | 271 bp | Primer Start | 143994 | Primer End | 144264 |
| FORWARD_PRIMER_3 | |||||||||||||
| ATACGGATCAGCCCCTTCTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCGTGGGGTTACCATTATCC | |||||||||||||
| Tm_F_3 | 59.925 | Size_F_3 | 20 | Tm_R_3 | 59.505 | Size_R_3 | 20 | PRODUCT Size | 204 bp | Primer Start | 143888 | Primer End | 144091 |