Primers Available
Organism:Silene noctiflora mitochondrion chromosome 19
Repeat:(AAG)4aatgtggtgcaggaggtgaaagcagaaagtggcattcttagattagacaagcatagttgaataaaagcaagcaagaaggaattccaactcaagtcc(TAGGG)3
Start:88973 End:89095
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCCGAGTCGTCCAATCATAG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTGATTGCTCGCTGAATGAC | |||||||||||||
| Tm_F_1 | 60.474 | Size_F_1 | 20 | Tm_R_1 | 59.955 | Size_R_1 | 20 | PRODUCT Size | 258 bp | Primer Start | 88908 | Primer End | 89165 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCCGAGTCGTCCAATCATAG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCGCTGAATGACCACCACTA | |||||||||||||
| Tm_F_2 | 60.474 | Size_F_2 | 20 | Tm_R_2 | 60.263 | Size_R_2 | 20 | PRODUCT Size | 250 bp | Primer Start | 88908 | Primer End | 89157 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCCGAGTCGTCCAATCATAG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CGCTGAATGACCACCACTAA | |||||||||||||
| Tm_F_3 | 60.474 | Size_F_3 | 20 | Tm_R_3 | 59.716 | Size_R_3 | 20 | PRODUCT Size | 249 bp | Primer Start | 88908 | Primer End | 89156 |