Primers Available
Organism:Silene conica mitochondrion chromosome 79
Repeat:(AGTA)3
Start:54297 End:54308
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGGGATGTGGTGATTCTTCC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GATGGGCAGGCTCTAGATGA | |||||||||||||
| Tm_F_1 | 60.725 | Size_F_1 | 20 | Tm_R_1 | 60.326 | Size_R_1 | 20 | PRODUCT Size | 268 bp | Primer Start | 54245 | Primer End | 54512 |
| FORWARD_PRIMER_2 | |||||||||||||
| AAACGAATTCACACGGGCTA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GTTGGAAATTGCAAGGAACC | |||||||||||||
| Tm_F_2 | 60.502 | Size_F_2 | 20 | Tm_R_2 | 59.41 | Size_R_2 | 20 | PRODUCT Size | 196 bp | Primer Start | 54177 | Primer End | 54372 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGGGATGTGGTGATTCTTCC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ATGCTTCGTCAAGAACCTCG | |||||||||||||
| Tm_F_3 | 60.725 | Size_F_3 | 20 | Tm_R_3 | 60.397 | Size_R_3 | 20 | PRODUCT Size | 224 bp | Primer Start | 54245 | Primer End | 54468 |