Primers Available
Organism:Cramptonomyia spenceri mitochondrion
Repeat:(TAAA)3
Start:9826 End:9837
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AAAGCCCCCTCACAAACTCT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTTTGATTTACAAGACCAATGTTTT | |||||||||||||
| Tm_F_1 | 60.11 | Size_F_1 | 20 | Tm_R_1 | 58.188 | Size_R_1 | 25 | PRODUCT Size | 262 bp | Primer Start | 9753 | Primer End | 10014 |
| FORWARD_PRIMER_2 | |||||||||||||
| AAATCCCCAATCCTAAAGCC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTTTGATTTACAAGACCAATGTTTT | |||||||||||||
| Tm_F_2 | 59.27 | Size_F_2 | 20 | Tm_R_2 | 58.188 | Size_R_2 | 25 | PRODUCT Size | 276 bp | Primer Start | 9739 | Primer End | 10014 |
| FORWARD_PRIMER_3 | |||||||||||||
| AAAGCCCCCTCACAAACTCT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AATTTTGATTTACAAGACCAATGTTTT | |||||||||||||
| Tm_F_3 | 60.11 | Size_F_3 | 20 | Tm_R_3 | 59.564 | Size_R_3 | 27 | PRODUCT Size | 264 bp | Primer Start | 9753 | Primer End | 10016 |