Primers Available
Organism:Cramptonomyia spenceri mitochondrion
Repeat:(T)10
Start:11014 End:11023
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GCCTTCGACAGAATTAGCCA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGGGTGTATAGGAATGACCCA | |||||||||||||
| Tm_F_1 | 60.352 | Size_F_1 | 20 | Tm_R_1 | 60.812 | Size_R_1 | 21 | PRODUCT Size | 149 bp | Primer Start | 10933 | Primer End | 11081 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCAACCCCCTCCAATATTTC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGGGTGTATAGGAATGACCCA | |||||||||||||
| Tm_F_2 | 60.37 | Size_F_2 | 20 | Tm_R_2 | 60.812 | Size_R_2 | 21 | PRODUCT Size | 269 bp | Primer Start | 10813 | Primer End | 11081 |
| FORWARD_PRIMER_3 | |||||||||||||
| GCCTTCGACAGAATTAGCCA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CCGATTAATCATGTAGGGGTG | |||||||||||||
| Tm_F_3 | 60.352 | Size_F_3 | 20 | Tm_R_3 | 59.168 | Size_R_3 | 21 | PRODUCT Size | 164 bp | Primer Start | 10933 | Primer End | 11096 |