Primers Available
Organism:Cramptonomyia spenceri mitochondrion
Repeat:(AATA)3
Start:9251 End:9262
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AGGTGCCTCAACATGTGCTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTTAGGGTGGGGGTATCAGC | |||||||||||||
| Tm_F_1 | 60.721 | Size_F_1 | 20 | Tm_R_1 | 61.061 | Size_R_1 | 20 | PRODUCT Size | 253 bp | Primer Start | 9048 | Primer End | 9300 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGCCTCAACATGTGCTTTTG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTTAGGGTGGGGGTATCAGC | |||||||||||||
| Tm_F_2 | 60.844 | Size_F_2 | 20 | Tm_R_2 | 61.061 | Size_R_2 | 20 | PRODUCT Size | 250 bp | Primer Start | 9051 | Primer End | 9300 |
| FORWARD_PRIMER_3 | |||||||||||||
| AGGTGCCTCAACATGTGCTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTTTAGGGTGGGGGTATCAG | |||||||||||||
| Tm_F_3 | 60.721 | Size_F_3 | 20 | Tm_R_3 | 58.76 | Size_R_3 | 20 | PRODUCT Size | 254 bp | Primer Start | 9048 | Primer End | 9301 |