Primers Available
Organism:Cramptonomyia spenceri mitochondrion
Repeat:(A)10
Start:7121 End:7130
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCATCTAAGCATTCTCCCCC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CTTGGCTTTGTGCGGAATAC | |||||||||||||
| Tm_F_1 | 60.787 | Size_F_1 | 20 | Tm_R_1 | 60.637 | Size_R_1 | 20 | PRODUCT Size | 280 bp | Primer Start | 6953 | Primer End | 7232 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCATCTAAGCATTCTCCCCC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCTTTGTGCGGAATACCTTT | |||||||||||||
| Tm_F_2 | 60.787 | Size_F_2 | 20 | Tm_R_2 | 59.229 | Size_R_2 | 20 | PRODUCT Size | 276 bp | Primer Start | 6953 | Primer End | 7228 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCATCTAAGCATTCTCCCCC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGCTTTGTGCGGAATACCT | |||||||||||||
| Tm_F_3 | 60.787 | Size_F_3 | 20 | Tm_R_3 | 60.088 | Size_R_3 | 19 | PRODUCT Size | 277 bp | Primer Start | 6953 | Primer End | 7229 |