Primers Available
Organism:Cramptonomyia spenceri mitochondrion
Repeat:(A)10
Start:6581 End:6590
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCTCCTCAACATCTTCAATGTCA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GATGAAGAGAATTTTTAGGGGG | |||||||||||||
| Tm_F_1 | 59.726 | Size_F_1 | 23 | Tm_R_1 | 58.152 | Size_R_1 | 22 | PRODUCT Size | 201 bp | Primer Start | 6496 | Primer End | 6696 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCTCCTCAACATCTTCAATGTCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGATGAAGAGAATTTTTAGGGG | |||||||||||||
| Tm_F_2 | 59.726 | Size_F_2 | 23 | Tm_R_2 | 58.152 | Size_R_2 | 22 | PRODUCT Size | 202 bp | Primer Start | 6496 | Primer End | 6697 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCTCCTCAACATCTTCAATGTCA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGGGGCAATCTTTATATCAGT | |||||||||||||
| Tm_F_3 | 59.726 | Size_F_3 | 23 | Tm_R_3 | 57.085 | Size_R_3 | 21 | PRODUCT Size | 183 bp | Primer Start | 6496 | Primer End | 6678 |