Primers Available
Organism:Ptychoptera sp. ATB-2011 mitochondrion
Repeat:(TTA)4
Start:669 End:680
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GCCCCTTTCCACTTTTGATT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTCCAGCTAATATTCATCCTAAATG | |||||||||||||
| Tm_F_1 | 60.299 | Size_F_1 | 20 | Tm_R_1 | 57.935 | Size_R_1 | 25 | PRODUCT Size | 247 bp | Primer Start | 538 | Primer End | 784 |
| FORWARD_PRIMER_2 | |||||||||||||
| GCCCCTTTCCACTTTTGATT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTTGATTTAATCCTCTTAAAGATCCA | |||||||||||||
| Tm_F_2 | 60.299 | Size_F_2 | 20 | Tm_R_2 | 58.731 | Size_R_2 | 26 | PRODUCT Size | 184 bp | Primer Start | 538 | Primer End | 721 |
| FORWARD_PRIMER_3 | |||||||||||||
| GCCCCTTTCCACTTTTGATT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTGATTTAATCCTCTTAAAGATCCA | |||||||||||||
| Tm_F_3 | 60.299 | Size_F_3 | 20 | Tm_R_3 | 57.566 | Size_R_3 | 25 | PRODUCT Size | 183 bp | Primer Start | 538 | Primer End | 720 |