Primers Available
Organism:Pielomastax zhengi mitochondrion
Repeat:(TTAT)3
Start:10276 End:10287
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGAAACTTCAGTAATGGGAAATGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGGTGTATAATTCGTTGGGG | |||||||||||||
| Tm_F_1 | 59.878 | Size_F_1 | 24 | Tm_R_1 | 59.019 | Size_R_1 | 20 | PRODUCT Size | 208 bp | Primer Start | 10174 | Primer End | 10381 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGAAACTTCAGTAATGGGAAATGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AGAGATGTTCATTGGTGTCGG | |||||||||||||
| Tm_F_2 | 59.878 | Size_F_2 | 24 | Tm_R_2 | 59.981 | Size_R_2 | 21 | PRODUCT Size | 266 bp | Primer Start | 10174 | Primer End | 10439 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGAAACTTCAGTAATGGGAAATGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GAGATGTTCATTGGTGTCGG | |||||||||||||
| Tm_F_3 | 59.878 | Size_F_3 | 24 | Tm_R_3 | 58.933 | Size_R_3 | 20 | PRODUCT Size | 265 bp | Primer Start | 10174 | Primer End | 10438 |