Primers Available
Organism:Pielomastax zhengi mitochondrion
Repeat:(AAAAT)3
Start:13252 End:13266
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AATTCAGTGAGCAGGTAAGACTTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GCATATAGGCTTTAAATCAGCCA | |||||||||||||
| Tm_F_1 | 57.761 | Size_F_1 | 24 | Tm_R_1 | 59.655 | Size_R_1 | 23 | PRODUCT Size | 269 bp | Primer Start | 13189 | Primer End | 13457 |
| FORWARD_PRIMER_2 | |||||||||||||
| ATTATGCTACCTTCGCACGG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ATGATTTTAGGTTGTTAATTGAGATTT | |||||||||||||
| Tm_F_2 | 60.117 | Size_F_2 | 20 | Tm_R_2 | 57.124 | Size_R_2 | 27 | PRODUCT Size | 246 bp | Primer Start | 13144 | Primer End | 13389 |
| FORWARD_PRIMER_3 | |||||||||||||
| ATTATGCTACCTTCGCACGG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AATGATTTTAGGTTGTTAATTGAGATT | |||||||||||||
| Tm_F_3 | 60.117 | Size_F_3 | 20 | Tm_R_3 | 57.124 | Size_R_3 | 27 | PRODUCT Size | 247 bp | Primer Start | 13144 | Primer End | 13390 |