Primers Available
Organism:Pseudotsuga sinensis var. wilsoniana chloroplast
Repeat:(TAA)4aatagaaaaaaaa(G)10
Start:45684 End:45718
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCCAGCCAATGATGATGGTA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTAGAGCAGCCAACCAAACC | |||||||||||||
| Tm_F_1 | 59.883 | Size_F_1 | 20 | Tm_R_1 | 60.249 | Size_R_1 | 20 | PRODUCT Size | 251 bp | Primer Start | 45558 | Primer End | 45808 |
| FORWARD_PRIMER_2 | |||||||||||||
| CGTGGATATCCCAATTCCAG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTAGAGCAGCCAACCAAACC | |||||||||||||
| Tm_F_2 | 60.148 | Size_F_2 | 20 | Tm_R_2 | 60.249 | Size_R_2 | 20 | PRODUCT Size | 266 bp | Primer Start | 45543 | Primer End | 45808 |
| FORWARD_PRIMER_3 | |||||||||||||
| GATATCCCAATTCCAGCCAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTAGAGCAGCCAACCAAACC | |||||||||||||
| Tm_F_3 | 59.722 | Size_F_3 | 20 | Tm_R_3 | 60.249 | Size_R_3 | 20 | PRODUCT Size | 262 bp | Primer Start | 45547 | Primer End | 45808 |