Primers Available
Organism:Pseudotsuga sinensis var. wilsoniana chloroplast
Repeat:(T)11
Start:20986 End:20996
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTTCGCCGCAATAAGAAACT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CGTACATTGCATTTGCAGG | |||||||||||||
| Tm_F_1 | 59.853 | Size_F_1 | 20 | Tm_R_1 | 58.716 | Size_R_1 | 19 | PRODUCT Size | 280 bp | Primer Start | 20892 | Primer End | 21171 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTCCGTTACAAGGAACTCGAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTATTGCCGAACCTAAAGCG | |||||||||||||
| Tm_F_2 | 58.707 | Size_F_2 | 21 | Tm_R_2 | 60.218 | Size_R_2 | 20 | PRODUCT Size | 265 bp | Primer Start | 20925 | Primer End | 21189 |
| FORWARD_PRIMER_3 | |||||||||||||
| CACTGTCACTTCCGTTACAAGG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTATTGCCGAACCTAAAGCG | |||||||||||||
| Tm_F_3 | 59.696 | Size_F_3 | 22 | Tm_R_3 | 60.218 | Size_R_3 | 20 | PRODUCT Size | 274 bp | Primer Start | 20916 | Primer End | 21189 |