Primers Available
Organism:Pseudotsuga sinensis var. wilsoniana chloroplast
Repeat:(T)10
Start:73455 End:73464
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCTTTGCAAGAAGGATGGCT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCATTGAAATGATCTCCGCT | |||||||||||||
| Tm_F_1 | 59.955 | Size_F_1 | 20 | Tm_R_1 | 60.036 | Size_R_1 | 20 | PRODUCT Size | 196 bp | Primer Start | 73378 | Primer End | 73573 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCTTGGTGTTCGGAAAAGAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CCATTGAAATGATCTCCGCT | |||||||||||||
| Tm_F_2 | 60.081 | Size_F_2 | 20 | Tm_R_2 | 60.036 | Size_R_2 | 20 | PRODUCT Size | 216 bp | Primer Start | 73358 | Primer End | 73573 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCTTGGTGTTCGGAAAAGAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ACCTCATACGGCTCCTCCTT | |||||||||||||
| Tm_F_3 | 60.081 | Size_F_3 | 20 | Tm_R_3 | 60.096 | Size_R_3 | 20 | PRODUCT Size | 173 bp | Primer Start | 73358 | Primer End | 73530 |