Primers Available
Organism:Cucumis sativus mitochondrion chromosome 3
Repeat:(TTCA)3ttacattaattcaagcaaagaaattgaaaaaatcataagttcagaactggtagttcttgctttctctctgaa(TCT)4
Start:5121 End:5216
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GCCATTGCTTTGGCTTAGAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AAAGCGATTCCTCTCTGGGT | |||||||||||||
| Tm_F_1 | 60.343 | Size_F_1 | 20 | Tm_R_1 | 60.212 | Size_R_1 | 20 | PRODUCT Size | 207 bp | Primer Start | 5059 | Primer End | 5265 |
| FORWARD_PRIMER_2 | |||||||||||||
| AAGGCGTAAAAGTTGCTTGC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AAAGCGATTCCTCTCTGGGT | |||||||||||||
| Tm_F_2 | 59.541 | Size_F_2 | 20 | Tm_R_2 | 60.212 | Size_R_2 | 20 | PRODUCT Size | 230 bp | Primer Start | 5036 | Primer End | 5265 |
| FORWARD_PRIMER_3 | |||||||||||||
| GCCATTGCTTTGGCTTAGAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGCGATTCCTCTCTGGGTCT | |||||||||||||
| Tm_F_3 | 60.343 | Size_F_3 | 20 | Tm_R_3 | 60.358 | Size_R_3 | 20 | PRODUCT Size | 205 bp | Primer Start | 5059 | Primer End | 5263 |