Primers Available
Organism:Cucumis sativus mitochondrion chromosome 3
Repeat:(TCGA)3
Start:31991 End:32002
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AGGAGTTCTTGTGGGAGGCT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TAAGCTCTCCCGGATGATTG | |||||||||||||
| Tm_F_1 | 60.252 | Size_F_1 | 20 | Tm_R_1 | 60.17 | Size_R_1 | 20 | PRODUCT Size | 245 bp | Primer Start | 31870 | Primer End | 32114 |
| FORWARD_PRIMER_2 | |||||||||||||
| CGAGTCTGGCAGAAGGAGAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TAAGCTCTCCCGGATGATTG | |||||||||||||
| Tm_F_2 | 59.555 | Size_F_2 | 20 | Tm_R_2 | 60.17 | Size_R_2 | 20 | PRODUCT Size | 276 bp | Primer Start | 31839 | Primer End | 32114 |
| FORWARD_PRIMER_3 | |||||||||||||
| AGGAGTTCTTGTGGGAGGCT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CCGCGGGATTATCTCTTTCT | |||||||||||||
| Tm_F_3 | 60.252 | Size_F_3 | 20 | Tm_R_3 | 60.542 | Size_R_3 | 20 | PRODUCT Size | 200 bp | Primer Start | 31870 | Primer End | 32069 |