Primers Available
Organism:Cucumis sativus mitochondrion chromosome 3
Repeat:(AGTC)3
Start:14410 End:14421
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTCCGGAAGAATGGAATCTG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GCCCTCCCAACTTGGATTAT | |||||||||||||
| Tm_F_1 | 60.006 | Size_F_1 | 20 | Tm_R_1 | 60.152 | Size_R_1 | 20 | PRODUCT Size | 158 bp | Primer Start | 14343 | Primer End | 14500 |
| FORWARD_PRIMER_2 | |||||||||||||
| CGTAAGAGCTCCCCTTTCCT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCCCTCCCAACTTGGATTAT | |||||||||||||
| Tm_F_2 | 59.842 | Size_F_2 | 20 | Tm_R_2 | 60.152 | Size_R_2 | 20 | PRODUCT Size | 232 bp | Primer Start | 14269 | Primer End | 14500 |
| FORWARD_PRIMER_3 | |||||||||||||
| GCTCCCCTTTCCTTCTTGTC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GCCCTCCCAACTTGGATTAT | |||||||||||||
| Tm_F_3 | 60.19 | Size_F_3 | 20 | Tm_R_3 | 60.152 | Size_R_3 | 20 | PRODUCT Size | 225 bp | Primer Start | 14276 | Primer End | 14500 |