Primers Available
Organism:Cucumis sativus mitochondrion chromosome 3
Repeat:(AGA)4
Start:4024 End:4035
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCAGAAGGCGCAAAGAATTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCAGGTCATCAGCCTTCAAT | |||||||||||||
| Tm_F_1 | 59.96 | Size_F_1 | 20 | Tm_R_1 | 60.073 | Size_R_1 | 20 | PRODUCT Size | 264 bp | Primer Start | 3923 | Primer End | 4186 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCAGAAGGCGCAAAGAATTT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGGATGAAATAGGCCAGGTC | |||||||||||||
| Tm_F_2 | 59.96 | Size_F_2 | 20 | Tm_R_2 | 59.894 | Size_R_2 | 20 | PRODUCT Size | 277 bp | Primer Start | 3923 | Primer End | 4199 |
| FORWARD_PRIMER_3 | |||||||||||||
| AAGCGAGAAGGTGCAAAGAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GCTTGCTGCTTAATCCCTTG | |||||||||||||
| Tm_F_3 | 60.132 | Size_F_3 | 20 | Tm_R_3 | 59.982 | Size_R_3 | 20 | PRODUCT Size | 267 bp | Primer Start | 3992 | Primer End | 4258 |