Primers Available
Organism:Cucumis sativus mitochondrion chromosome 3
Repeat:(AG)6
Start:4500 End:4511
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ATATCTTTCTTGGACCGCCC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AAGGGACCAAGGTGTGAGTG | |||||||||||||
| Tm_F_1 | 60.286 | Size_F_1 | 20 | Tm_R_1 | 60.002 | Size_R_1 | 20 | PRODUCT Size | 170 bp | Primer Start | 4475 | Primer End | 4644 |
| FORWARD_PRIMER_2 | |||||||||||||
| ATATCTTTCTTGGACCGCCC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CTTGGATCTCAGCCGCTAAC | |||||||||||||
| Tm_F_2 | 60.286 | Size_F_2 | 20 | Tm_R_2 | 59.978 | Size_R_2 | 20 | PRODUCT Size | 253 bp | Primer Start | 4475 | Primer End | 4727 |
| FORWARD_PRIMER_3 | |||||||||||||
| GCATATCTTTCTTGGACCGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AAGGGACCAAGGTGTGAGTG | |||||||||||||
| Tm_F_3 | 59.674 | Size_F_3 | 20 | Tm_R_3 | 60.002 | Size_R_3 | 20 | PRODUCT Size | 172 bp | Primer Start | 4473 | Primer End | 4644 |