Primers Available
Organism:Cucumis sativus mitochondrion chromosome 2
Repeat:(TTC)4
Start:111 End:122
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CGCGTAGTGCTTTCTTTCAG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CTCGGAAGCAAGGAGAGATG | |||||||||||||
| Tm_F_1 | 58.87 | Size_F_1 | 20 | Tm_R_1 | 60.088 | Size_R_1 | 20 | PRODUCT Size | 167 bp | Primer Start | 35 | Primer End | 201 |
| FORWARD_PRIMER_2 | |||||||||||||
| CGCGTAGTGCTTTCTTTCAG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ATTACGGGTTACGGGGAATC | |||||||||||||
| Tm_F_2 | 58.87 | Size_F_2 | 20 | Tm_R_2 | 59.909 | Size_R_2 | 20 | PRODUCT Size | 267 bp | Primer Start | 35 | Primer End | 301 |
| FORWARD_PRIMER_3 | |||||||||||||
| CGCGTAGTGCTTTCTTTCAG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGGCTTATTACGGGTTACGG | |||||||||||||
| Tm_F_3 | 58.87 | Size_F_3 | 20 | Tm_R_3 | 59.847 | Size_R_3 | 20 | PRODUCT Size | 273 bp | Primer Start | 35 | Primer End | 307 |