Primers Available
Organism:Cucumis sativus mitochondrion chromosome 2
Repeat:(TCT)4
Start:25175 End:25186
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCTGTCGCTCTTTCTTAGCCA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TACTCTGCCAGACGGAGACC | |||||||||||||
| Tm_F_1 | 60.277 | Size_F_1 | 21 | Tm_R_1 | 60.408 | Size_R_1 | 20 | PRODUCT Size | 208 bp | Primer Start | 25033 | Primer End | 25240 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCTGTCGCTCTTTCTTAGCCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CCATCCCTTGATACTCTGCC | |||||||||||||
| Tm_F_2 | 60.277 | Size_F_2 | 21 | Tm_R_2 | 59.508 | Size_R_2 | 20 | PRODUCT Size | 219 bp | Primer Start | 25033 | Primer End | 25251 |
| FORWARD_PRIMER_3 | |||||||||||||
| CTGTCGCTCTTTCTTAGCCA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TACTCTGCCAGACGGAGACC | |||||||||||||
| Tm_F_3 | 58.405 | Size_F_3 | 20 | Tm_R_3 | 60.408 | Size_R_3 | 20 | PRODUCT Size | 207 bp | Primer Start | 25034 | Primer End | 25240 |